日韩精品人妻一区二区中文,五月婷婷综合在线视频,A久久精品国产精品亚洲,亚州中文精品有码视频在线,精品1区2区3区产品乱码,色哟哟免费观看视频入口,婷婷射精AV这里只有精品,2024日产乱码国产,国产精品三级一区二区,艳妇乳肉豪妇荡乳AV

Size (kb): 4.320

Vector: pFN476 (plasmid)

Promoters: Promoter lac

Construction: pGEM3Z, pKA101-6

Marker(s):ampR

Construct size (kb): 4.320

Features: insert detection: lacZ'
marker(s): ampR
other: par
promoter: lac
promoter for expression: T7
promoter for in vitro transcription: SP6
replicon: pSC101
replicon: rep (pSC101)
MCS: pGEM-3Z; HindIII...EcoRI
restriction site: BamHI
coding sequence: tnpR

expression vector T7 promoter

vector permitting RNA synthesis in vitro

vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase

vector with low copy number

Restriction digests of the clone give the following sizes (kb): EcoRI--4.2; HindIII--4.2; PstI--3.1, 1.1.

Low copy number vector to express transcripts in vivo from the T7 promoter and to minimize toxic gene effects.

Plasmid contains the pSC101 replicon and is maintained at 1-5 copies per cell.

Low copy number minimizes the effect of uninduced expression of T7 RNA polymerase, reducing transcription of potentially toxic inserts.

T7 RNA polymerase can also be provided by infection of F' hosts with M13mGP1-2 (ATCC 40303).

Temperature: 37.0°C