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Size (kb): 8.3999996185302730

Vector: YEp364 (plasmid)

Promoters: Promoter none

Construction: pNM481, YEp351

Marker(s):LEU2,ampR

Construct size (kb): 8.399999618530273

Features: insert detection: lacZ
marker(s): ampR, LEU2
promoter: none
replicon: pMB1, 2 micron

YE-type (episomal) shuttle vector

promoter-cloning vector

shuttle vector

The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-1; SmaI-2; BamHI-2; SalI-2; PstI-3; HindIII-1. The EcoRI site is not unique.

Restriction digests of the clone give the following sizes (kb): HindIII--8.4; BamHI--8.4; SalI--8.4; PstI--8.4; EcoRI--4.25, 4.15.

One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37728 - 37730) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.

The sequence and reading frame of the multiple cloning sequence is: 5' GAA TTC CCG GGG ATC CGT CGA CCT GCA GCC AAG CTT GCT CCC 3'.

Temperature: 37.0°C