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phTGF1-10-925
phTGF1-10-925
規(guī)格:
貨期:
編號:B219526
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 phTGF1-10-925
商品貨號 B219526
Designations phTGF1-10-925
GenBank Number

X70340

Species Homo sapiens, human
Vector
Construct size (kb): 4.300000190734863
Insert
DNA: cDNA
Insert lengths(kb): 0.925000011920929
Tissue: kidney
Gene product: transforming growth factor, alpha(transforming growth factor alpha) [TGFA]
Alleles: A1, A2, B1, B2
Insert Size (kb) 0.925
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
The insert contains the following restriction sites, in bp from the 5' end of the insert: PstI (167), PstI (234), RsaI (285), XmnI (590).
Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 0.9; BglI--1.5, 1.1 (doublet), 0.3; SphI--2.9, 1.1; BamHI--4.3; PvuI--4.3.
The insert includes 49 bp of 5' untranslated, 480 bp encoding transforming growth factor alpha precursor, and 391 bp 3' untranslated.
The RsaI polymorphism is in linkage disequilibrium with a BamHI polymorphism detected by phTGF1-10-3350, (r=0.55, p=0.03).
Enzyme(s) not detecting polymorphism: BclII, BglII, EcoRI, EcoRV, HaeIII, HindIII, HinfI, KpnI, MspI, PstI, PvuII, SacI, XbaI, XhoI.
References

Tricoli JV, et al. The gene for human transforming growth factor alpha is on the short arm of chromosome 2. Cytogenet. Cell Genet. 42: 94-98, 1986. PubMed: 3459638

Murray JC, et al. RFLPs for transforming growth factor alpha (TGFA) gene at 2p13. Nucleic Acids Res. 14: 7136, 1986. PubMed: 2876411

Murray JC, et al. Linkage of an autosomal dominant clefting syndrome (Van der Woude) to loci on chromosome 1q. Am. J. Hum. Genet. 46: 486-491, 1990. PubMed: 2309700

Graeme I Bell, personal communication

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