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pHM 2.6 [pHM2.6]
pHM 2.6 [pHM2.6]
規(guī)格:
貨期:
編號:B217143
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 pHM 2.6 [pHM2.6]
商品貨號 B217143
Designations pHM 2.6 [pHM2.6]
Species Homo sapiens, human
Depositors D Stehelin
Applications
Enzyme(s) not detecting polymorphism: HindIII, BglII, BamHI, SstI, PstI. Tested against 5 unrelated individuals.
Vector
Construct size (kb): 7.099999904632568
Insert
DNA: genomic
Insert lengths(kb): 2.599999904632568
Tissue: fetal liver
Gene product: avian myeloblastosis viral (v-myb) oncogene homolog [MYB]
Alleles: A2, A1
Insert Size (kb) 2.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Insert contains HindIII, SstI, and 2 XbaI sites.
Restriction digests of the clone give the following sizes (kb): BamHI--7.1; AvaI--7.1; HindIII--4.5, 2.6; PstI--4.5, 1.8, 0.8; EcoRI--4.5, 2.6.
Cross-hybridizes with chicken myb sequences.
EcoRI RFLP demonstrated co-dominant segregation in 1 informative family of 9. Allele A1 is amplified in ML3 and COLO 210 cell lines.
Enzyme(s) not detecting polymorphism: HindIII, BglII, BamHI, SstI, PstI. Tested against 5 unrelated individuals.
References

Hall JM, et al. Oncogenes and human breast cancer. Am. J. Hum. Genet. 44: 577-584, 1989. PubMed: 2564734

Leprince D, et al. The human DNA locus related to the oncogene myb of avian myeloblastosis virus (AMV): molecular cloning and structural characterization. EMBO J. 2: 1073-1078, 1983. PubMed: 6194989

Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217

Dozier C, et al. EcoRI RFLP linked to the human myb gene. Nucleic Acids Res. 14: 1928, 1986. PubMed: 3005993

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